Fig. 5.

Infection with Ad vectors encoding K^b mutants drives CD8 T cell expansion and differentiation in vivo. (A–D) B6 CD8-KO recipients were transferred with donor CFSE labeled CD45.1 congenic B6 CD8 T cells before being infected with Ad-K^b, Ad-Q72W, or Ad-G162W by intradermal injection. Control mice were uninfected or infected with a control Ad encoding a GFP-Luciferase fusion protein (Ad-control). Cells were processed from the dLN at 4 dpi. Flow cytometry was used to quantify activated transferred CD8 T cells that had undergone cell division as measured by expression of CD44 and dilution of CFSE (A) and effector differentiation as measured by expression of CD44 and down-regulation of CD62L (B). Absolute counts of CD44⁺ fully divided (C) and CD44⁺CD62L⁻ (D) donor CD8 T cells are shown for each of the Ad infections tested. Bars represent means ± SD of n = 3 independent experiments. Statistical significance was calculated using repeated measures one-way ANOVA with Dunnett’s multiple comparisons test.