Fig. 2.

Proteomics experiments with cell-free extracts reveal a putative desulfonating GRE. (A and B) Proteins encoded by the taurine and isethionate utilization gene clusters, including the predicted isethionate GRE (IslA) and GRE-activase (IslB) components, are specifically and strongly expressed during growth with taurine (B. wadsworthia) and isethionate (B. wadsworthia, Desulfovibrio strains). Constitutively expressed proteins for, for example, sulfite reduction (Dsr) and metabolism of acetyl-CoA (Pta and AckA), are shown for comparison. IMG locus tag numbers are given; their prefixes are shown in Fig. 1C. Shown are results of representative total proteomic analyses replicated at least twice with extracts prepared from independent growth experiments.