Table 1. Primers of target genes.
Gene | Forward (5′–3′) | Reverse (5′–3′) |
---|---|---|
GAPDH | CCATCAACGACCCCTTCATT | ATTCTCAGCCTTGACTGTGC |
Aggrecan | GCCTGCAAGGGAAATGTGTG | GGCGGAAAGATTTGCCGTTAG |
Collagen II | GACCTCCGGCTCCTGCTCCTCTTAG | GACAGCACTCGCCCTCCCGTTTTTG |
Bax | GGCGAATTGGCGATGAACTG | CCCAGTTGAAGTTGCCGTCT |
Bcl-2 | CGCCCGCTGTGCACCGAGA | CACAATCCTCCCCCAGTTCACC |
Caspase-3 | TACCCTGAAATGGGCTTGTGT | GTTAACACGAGTGAGGATGTG |
TIMP-1 | ACCTGGTTAAGGGCTAAATTCATG | ATTTCCACAGCGTCGAATC |
TIMP-3 | CCTTTGGCACTCTGGTCTACACT | CTTTCAGAGGCTTCCGTGTGA |
MMP-3 | AAAGAACCCGCTGAGAGCAG | AACCTCCATGCCAGCATCTT |
ADAMTS-4 | CGTTCCGCTCCTGTAACACT | TTGAAGAGGTCGGTTCGGTG |