Fig. 1.
Region-specific knockdown of the transgene expression in 14-3-3 FKO mice. a Representative image of YFP (green) expression in tsA 201 cells transfected with YFP-difopein cDNA. b Representative images of reduced YFP expression in cells co-transfected with cDNAs of YFP-difopein and pSicoR-mCherry-shRNA. c Schematic map of recombinant adeno-associated viral (rAAV) vector used to co-express the shRNA against YFP (shRNA) and mCherry proteins. d Representative image of a sagittal section of a 14-3-3 FKO mouse brain injected with AAV-mCherry-shRNA (red) in both the prefrontal cortex (PFC) e and dorsal hippocampus (HP) f. Virus (red) and difopein transgene (green) expression were detected using fluorescent and confocal microscopy. There is minimal colocalization between red and green signals in the PFC (EI−III) or HP CA1 (FI−III), indicating a knockdown of difopein expression in AAV-infected cells. g, h Representative images of the PFC (GI−III) and the HP CA1 (HI−III) of the 14-3-3 FKO mice injected with the negative control (NC) AAV expressing a scrambled shRNA and mCherry. There is colocalization of red and green signals in both the PFC and HP regions. Scale bars represent 300 μm d and 100 μm e–h