Fig. 6.

Reduction of NMDA receptor subunits in the post-synaptic density induced by YFP-difopein expression. a Representative images of western blots from dorsal hippocampal PSD fractions of WT mice injected with either control virus (AAV-YFP) or AAV-YFP-difopein virus. b Compared with that of controls (n = 6 for all groups), there is a significant decrease in the levels of GluN1 (n = 6, F (1, 10) = 14.60, p = 0.012), GluN2A (n = 6, F(1, 10) = 12.50, p = 0.017) subunits and the levels of PSD95 (n = 6, F(1, 10) = 11.73, p = 0.019) in YFP-difopein injected mice. Blots were probed with GAPDH as a loading control and protein levels were normalized to that of WT control. Data are presented as mean ± S.E.M., with statistical significance *p < 0.05, two-tailed t-test