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. 2019 Jan 24;20(3):503. doi: 10.3390/ijms20030503

Figure 2.

Figure 2

The impact of mechanical loading on sensory neuropeptide receptor protein expression of RAW264.7 cells. Receptor protein expression of NK1R (A), CRLR (B) and Ramp1 (C) was analyzed using the Western Blotting of lysates from cells loaded for 4 h/day on 1, 2 and 3 consecutive days. Expression of β-actin served as endogenous loading control (=100% line). Mann–Whitney test * p < 0.05; ** p < 0.01; *** p < 0.001. n = 7–8; (D) Representative Western Blot pictures for the CRLR (~53 kDa), NK1R (~46 kDa), Ramp1 (~17 kDa) and β-actin (~37 kDa, endogenous control) of control cells and cells loaded for 1, 2 and 3 consecutive days (presenting 2 lanes for each condition). NK1R—neurokinin receptor 1, CRLR—calcitonin receptor-like receptor, Ramp1—receptor activity modifying protein 1.