Figure 2.

Effect of cirsiliol on migratory potential of B16F10 cells. (A) Wound healing assay showed reduction in the migratory property of FN⁺/Cir (10 µM/48 h)-treated B16F10 cells with respect to fibronectin (FN⁺) [20 µg/mL]-induced cells even after 24 h of monitoring. (B) The distance of wound closure (measured by image J software in pixels/cm) was much more in case of FN⁺/Cir (10 µM/48 h) treated B16F10 cells than FN⁺-induced cells. (C) Trans-well migration assay micrographs (400× magnification) showed compromised migratory property in FN⁺/Cir (10 µM/48 h)-treated B16F10 cells compared to FN⁺ B16F10 cells. (D) The percentage of cells which migrated across the Boyden’s chamber were significantly less in case of FN⁺/Cir (10 µM/48 h)-treated cells than the FN⁺-induced cells. Cir: cirsiliol; FN⁺: cells were seeded in pre-coated (cellular FN 20 μg/mL for 2 h at 37 °C) petri dishes and further incubated for 48 h in presence of 5% fetal bovine serum (FBS) for induction of EMT; FN⁺/Cir (10 µM/48 h): cells were seeded in pre-coated (cellular FN 20 μg/mL for 2 h at 37 °C) petri dishes and subjected to a non-cytotoxic dose of cirsiliol (10 µM) treatment for 48 h. All quantitative results are expressed as mean ± SD based on three replicates.