Figure 7.

Effect of cirsiliol on NF-κB signaling in B16F10 cells. (A) Micrographs of immunocytochemistry (400× magnification) exhibited reduced nuclear expression of NF-κB p65 in FN⁺/Cir (10 µM/48 h) treated cells than the FN⁺-induced cells. (B) Quantitative analysis of cells with positive nuclear expression of NF-κB p65. (C) Western blot images and (D) graphical representation of the normalized band intensities of nuclear NF-κB p65 in FN⁺/Cir (10 µM/48 h) treated cells in comparison to FN⁺ B16F10 cells. (E) mRNA images and (F) mean band intensities of mRNA of NF-κB p65 in FN⁺/Cir (10 µM/48 h)-treated cells in comparison to FN⁺ B16F10 cells. Cir: cirsiliol; FN⁺: cells were seeded in pre-coated (cellular FN 20 μg/mL for 2 h at 37 °C) petri dishes and further incubated for 48 h in presence of 5% FBS for induction of EMT; FN⁺/Cir (10 µM/48 h): cells were seeded in pre-coated (cellular FN 20 μg/mL for 2 h at 37 °C) petri dishes and subjected to a non-cytotoxic dose of cirsiliol (10 µM) treatment for 48 h, M: DNA step ladder 100 bp. All quantitative results are expressed as mean ± SD based on three replicates. Scanned blots of Figure 7 are showed in Figure S4.