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Binding of FITC/YT-16 peptide with PD-1 on T cells. T cells were incubated with 10, 100, and 1000 nM FITC/YT-16 peptide at 37 °C for 2 h. Binding of FITC/YT-16 with PD-1 was detected with flow cytometry technology. Isotype antibody was used as a control and to define the gate for positive fluorescence signal.
