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. 2019 Jan 24;20(3):506. doi: 10.3390/ijms20030506

Figure 6.

Figure 6

The effect of α5 integrin silencing on SVS-induced mRNA expression levels of osteogenic marker genes, ALP activity and calcium deposition in D1 cells. D1 cells (passage 9) transfected with nonspecific control oligonucleotides (mock group) or α5 integrin-specific siRNA (si-α5 group) were treated with SVS at concentrations of 0 (Ctrl), 0.25 and 0.5 µM for 3 days, and the medium was changed to osteoinduction for another 2 to 6 days. (A) The mRNA expression levels of the osteogenic marker genes (α5 integrin, Runx2, BMP-2, ALP, and OC) in the mock and si-α5 group groups at day 3. The gene expression levels are expressed relative to that in the mock group at day 1, which is defined as 1. The values are presented as the mean ± SD (n = 3), * p < 0.05 in comparison to the mock group. (B) ALP activity staining in the mock and si-α5 groups on day 2 after the medium was changed to osteoinduction medium (a). Blue: staining for ALP activity. Alizarin red S staining of calcium deposition in the mock and si-α5 groups on day 6 (b) and 8 (c) after the media was changed to osteoinduction medium. Red: Alizarin red S staining.