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. 2019 Jan 31;20(3):622. doi: 10.3390/ijms20030622

Figure 3.

Figure 3

Immunoblotting of the zebrafish ST8Sia VI-like recombinant protein produced in transfected COS-7 cells media. COS-7 cell culture media from pFLAG-CMV-9/Dre ST8Sia VI-like or mock transfected cells (48 h post-transfection) were treated (+) or not (−) with PNGase F and were subjected to SDS-PAGE under reducing conditions and Western blotting using the BioM2 anti-FLAG mAb. The position of the high range pre-stained SDS-PAGE standards are indicated in kDa on the left side. Lane 1 and 2: mock transfected cells; Lane 3 and 4: pFLAG-CMV-9/DreST8Sia VI-like transfected cells.