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. 2019 Jan 29;20(3):580. doi: 10.3390/ijms20030580

Figure 6.

Figure 6

eIF5B knock-down negatively regulates the expression of Bcl-xL and MG1. (A) U2OS cells were transfected with eIF5B-targeting or non-targeting control siRNAs followed by 12 h of mock- or infection with MG1 at MOI = 0.1. Western blot demonstrates the level of eIF5B, Bcl-xL, XIAP, MG1 and Tubulin (loading control) proteins. The expressions of Bcl-xL and XIAP proteins were quantified relative to Tubulin by densitometry (*** p = 0.0009; ns, non-significant). (B) Steady-state levels of Bcl-xL, XIAP mRNA and MG1 genomic RNA were measured by RT-qPCR in siCTRL or siEIF5B transfected cells after 12 hpi with MG1 or DMEM treatment. Ct values were normalized by the standard curve method and represented relative to the geometric mean of RPL13 A and RPL36A (**** p < 0.0001; ns, non-significant).