Figure 7.

eIF5B knock-down affects the rate of MG1 infection and cytotoxicity. (A) Top: western blot from [³⁵S]-methionine labeling translational assay, U2OS cells were transfected with siCTRL or siEIF5B, followed by MG1 infection at MOI = 0.1 for 12 and 15 h. Bottom: Densitometry of M protein signal relative to Coomassie blue stain demonstrates the rate of MG1 mRNA translation at the indicated time points (B) Top left panel: U2OS cells were treated as in (A) and the rate of MG1 infection was monitored using IncuCyte Zoom live cell imaging. Y axis represents the percentage of GFP-MG1 infected cell relative to cell confluency. Top right panel: Fold confluency of GFP-MG1 signal at 10 and 12 hpi. Bottom panel: MG1 cytotoxicity was compared between the previously described conditions by monitoring signals from Cytotox Red reagent using IncuCyte Zoom. Y axis represents the number of cells emitting fluorescent red relative to cell confluency.