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Identifying candidate aggrecanases in TS4/5∆cat mice. (a) Aggrecanase cleavage in TS-4/5∆cat femoral head cartilage explants, cultured untreated (lane 1) or in the presence of actinomycin D (lane 2), retinoic acid (lane 3) or both actinomycin D and retinoic acid together (lane 4) was analysed by FREEE 1467 neo-epitope Western blotting. (b) The results of the microarray studies, represented as a volcano plot, identify candidate proteinases for the novel aggrecanase.
