Table 1.
Virus Productivity.
| Optimization Study (Number) | Harvesting Time (h) | MOI | Harvesting Method | Total Virus Particles (IU), (Mean) | Virus Productivity (Number of Virus Particles/Cell), (Mean) |
|---|---|---|---|---|---|
| 1 (T-175) | 48 | 10 | Lysis A | 1.86 × 1011 | 5576 |
| 60 | 2.08 × 1011 | 6226 | |||
| 72 | 1.82 × 1011 | 5438 | |||
| 84 | 1.46 × 1011 | 4365 | |||
| 96 | 1.77 × 1011 | 5299 | |||
| 2 (T-175) | 66 | 10 | Lysis A | 2.47 × 1011 | 6682 |
| 72 | 1.63 × 1011 | 3779 | |||
| 78 | 2.19 × 1011 | 5083 | |||
| 84 | 1.92 × 1011 | 4476 | |||
| 90 | 1.90 × 1011 | 5124 | |||
| 3 (T-175) | 56 | 10 | Lysis A | 2.87 × 1011 | 7966 |
| 60 | 2.95 × 1011 | 8205 | |||
| 66 | 3.07 × 1011 | 8538 | |||
| 72 | 2.30 × 1011 | 6401 | |||
| 4 (T-175) | 66 | 1 | Lysis A | 6.39 × 1010 | 1494 |
| 5 | 2.17 × 1011 | 5085 | |||
| 20 | 3.06 × 1011 | 7153 | |||
| 50 | 3.30 × 1011 | 7715 | |||
| 100 | 1.85 × 1011 | 4338 | |||
| 5 (HYPERFlask) | 66 | 30 | Lysis A | 3.02 × 1012 | 14,559 |
| 6 (HYPERFlask) | 60 | TrypLE | 8.51 × 1011 | 2497 | |
| 66 | 6.96 × 1011 | 2044 | |||
| 72 | 7.40 × 1011 | 2172 | |||
| 96 | 8.43 × 1011 | 2475 | |||
| 7 (T-175 & HYPERFlask) | 66 | 30 | Lysis A T-175 |
3.72 × 1011 | 10,887 |
| Lysis B T-175 |
1.48 × 1011 | 4331 | |||
| Lysis C T-175 |
1.96 × 1011 | 5741 | |||
| Lysis D HYPERFlask |
4.36 × 1011 | 1279 | |||
| Lysis C HYPERFlask |
8.09 × 1011 | 2374 | |||
| Benzonase treatment (pellet 1) HYPERFlask |
4.35 × 1011 | 1275 | |||
| Benzonase treatment (pellet 2) HYPERFlask |
1.08 × 1011 | 315 |