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. 2019 Jan 31;20(3):621. doi: 10.3390/ijms20030621

Table 1.

Virus Productivity.

Optimization Study (Number) Harvesting Time (h) MOI Harvesting Method Total Virus Particles (IU), (Mean) Virus Productivity (Number of Virus Particles/Cell), (Mean)
1 (T-175) 48 10 Lysis A 1.86 × 1011 5576
60 2.08 × 1011 6226
72 1.82 × 1011 5438
84 1.46 × 1011 4365
96 1.77 × 1011 5299
2 (T-175) 66 10 Lysis A 2.47 × 1011 6682
72 1.63 × 1011 3779
78 2.19 × 1011 5083
84 1.92 × 1011 4476
90 1.90 × 1011 5124
3 (T-175) 56 10 Lysis A 2.87 × 1011 7966
60 2.95 × 1011 8205
66 3.07 × 1011 8538
72 2.30 × 1011 6401
4 (T-175) 66 1 Lysis A 6.39 × 1010 1494
5 2.17 × 1011 5085
20 3.06 × 1011 7153
50 3.30 × 1011 7715
100 1.85 × 1011 4338
5 (HYPERFlask) 66 30 Lysis A 3.02 × 1012 14,559
6 (HYPERFlask) 60 TrypLE 8.51 × 1011 2497
66 6.96 × 1011 2044
72 7.40 × 1011 2172
96 8.43 × 1011 2475
7 (T-175 & HYPERFlask) 66 30 Lysis A
T-175
3.72 × 1011 10,887
Lysis B
T-175
1.48 × 1011 4331
Lysis C
T-175
1.96 × 1011 5741
Lysis D
HYPERFlask
4.36 × 1011 1279
Lysis C
HYPERFlask
8.09 × 1011 2374
Benzonase treatment (pellet 1)
HYPERFlask
4.35 × 1011 1275
Benzonase treatment (pellet 2)
HYPERFlask
1.08 × 1011 315