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. 2019 Feb 3;20(3):660. doi: 10.3390/ijms20030660

Figure 2.

Figure 2

Cytotoxic activity of engineered anti-CD24-CAR-NK-92 cells is restricted to antigen-expressing cells (A,B). After lentiviral transduction of A2780 (6.6% CD24 positive) (A) and HEK-293T cells (CD24 negative) (B), cells were seeded in 96-well plates and co-incubated with the indicated NK cells at an E/T ratio of 5:1. The graphics illustrate the Fluoroskan results after 24 h incubation. * indicate p < 0.05 (unpaired t-test). Values represent the mean from two separate experiments, each containing three samples. (C) Serial photos of a co-culture of CD24 positive OVCAR3 cells (green) with CD24 negative HEK-293T cells (grey) under the fluorescence microscope. Target cells (OVCAR3 and HEK-293T) were mixed at a ratio of 1:1. Anti-CD24-CAR-NK-92 cells were then added with an E/T ratio of 1:1. Anti-CD24 NK-92 cells (red) specifically kill only CD24 positive OVCAR3 cells and later proliferate. Arrows point to engineered NK-92 cells. Time stamp indicates hh:mm:ss.