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(A) MSCs grown on Gp exhibited greater increases of Smad p1/5 and of the classical markers for osteogenic differentiation, RUNX2, OPN and OCN. The expressions of p-SMAD increased by >180% on Gp compared to PDMS alone. The treatment 50 µM of DMHI decreased the expression of all protein studies. (B) Relative quantification of all groups in the absence of inhibitors. Signal intensity is in arbitrary units.
