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. 2019 Jan 23;20(3):478. doi: 10.3390/ijms20030478

Figure 3.

Figure 3

GLP prevented PA-induced apoptosis in IPEC-J2 cells. Cells were exposed to 0.4 and 0.8 mg/mL GLP in presence of 600 μM PA for 24 h. (A) Quantification of IPEC-J2 apoptotic cells by flow cytometry. Four parts were observed: necrotic cells (upper left quadrants), late apoptotic and necrotic cells (upper right quadrants), viable cells (lower left quadrants), and early apoptotic cells (lower right quadrant). (B) The expressions of Bax, Bcl-2, Cyto c, caspase-3 (cas-3), and PARP were analyzed by Western blot. (C) The data were quantified and normalized to β-actin. Data shown are representative of three independent experiments (n = 3). A < 0.05 and a < 0.01 vs. control, B < 0.05 and b < 0.01 vs. PA alone.