Figure 4.

The effects GLP and PA on mitochondrial energy metabolism in IPEC-J2 cells. Cells were treated with 600 μM PA or/and GLP (0.4 and 0.8 mg/mL) for 24 h. (A) Citrate synthase activity was assayed using a citrate synthase assay kit and expressed as U/mg protein (n = 5). (B) Mitochondrial proteins were extracted using a mitochondria isolation kit after cells were lysed. The ATP level was determined by bioluminescence measurement and expressed as μmol/g protein (n = 5). (C) Representative Western blot of AMPK and p-AMPK. The data were quantified and normalized to β-actin. Data were presented as mean ± SE for three independent experiments, a < 0.01 vs. control, b < 0.01 vs. PA alone.