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. 2019 Jan 31;20(3):612. doi: 10.3390/ijms20030612

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression of differentiation markers in hBM-MSC following dexamethasone treatment as measured by Flow cytometer. The histogram is a representative example of the percentages of expression of various bone marrow stem cell and osteoblast differentiation markers, before and after treatment with dexamethasone. Control Osteoblasts were used as positive control. Control osteoblast and BM001 isolated hBM-MSC cells were incubated with anti ALP, CD105, CD44, CD90, Osteopontin, Osteonectin, Osteocalcin, and RUNX2 specific fluoresceinated antibodies and flow cytometry analysis was used to determine percent of expression of the different proteins before and after incubation with dexamethasone to study their differentiation capabilities.