Figure 2.

The Ca²⁺ response to manuka honey requires extracellular Ca²⁺ entry. (A) The Ca²⁺ response to 4% v/v manuka honey was abolished in Ca²⁺-free medium. Control cells, which were not exposed to the treatment, did not show any change in [Ca²⁺]_i. The arrow indicates the addition of manuka honey after 60 s. Data are means ± SEM of [Ca²⁺]_i traces recorded in different cells. Number of cells: CTRL: 20 cells from 2 exp; manuka honey: 40 cells from 3 exp; manuka honey w/o external Ca²⁺: 30 cells from 3 exp. (B) Mean ± SEM of the peak Ca²⁺ response recorded under the designated treatments. Number of cells as in A. Different letters above bars indicate statistical difference determined by One-way ANOVA followed by Dunnet post-test (p < 0.01).