Figure 2.

Differential glycomic profiling of tissue fragments obtained from medulla/Ulex europaeus agglutinin-I (UEA-I)(+) and cortex/UEA-I(−) regions by each method. Serial thymic sections were stained with hematoxylin or with UEA-I, and then subjected to LMD-LMA analysis by Methods 1, 2, and 3. (A) Whole section images after dissection (left panels) and representative images of tissue dissection at the areas indicated in the left panels (middle and right panels). Whole section images before or after tissue dissection with sample annotations are shown in Figure S5A. (B) Principle component analysis (PCA) score plot of glycomic profiles of tissue fragments obtained from the two regions by each method. (C) PCA score plot (left panel) and loading plot (right panel) of glycomic profiles of the medulla/UEA-I(+) tissue fragments. In the loading plot, the vector corresponding to UEA-I is highlighted in blue. (D) Relative UEA-I signal intensities of the medulla/UEA-I(+) tissue fragments. Asterisks indicate significant differences between groups (Tukey’s honestly significant difference, p < 0.05). Glycomic profile data (0.5 mm² equivalent; n = 6 each) used for these analyses are presented in Table S4.