The VEGFa and VEGFr-2 immunoblots. (A) Levels of VEGFa and VEGFr-2 in the IS+CP (I) and corpus plus cauda (CO+CD) epididymis (II) of NP and low-protein (LP) animals on PND 21. Representative blots showing the levels of the VEGF, VEGFr-2 and β-actin proteins (70 µg of protein) in 21-day animals (III). (B) Levels of VEGFa and VEGFr-2 in the IS+CP (I) and CO+CD epididymis (II) of NP and LP animals on PND 44. Representative blots showing the levels of the VEGF, VEGFr-2, and β-actin proteins (70 µg of protein) in 44-day animals (III). (C) Levels of VEGFa and VEGFr-2 in the IS+CP (I) and CO+CD epididymis (II) of NP and LP animals on PND 120. Representative blots showing the levels of the VEGF, VEGFr-2 and β-actin proteins (70 µg of protein) in 120-day animals (III). Data are presented as means ± S.E.M. * p < 0.05, Mann-Whitney test.