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. 2019 Feb 4;20(3):668. doi: 10.3390/ijms20030668

Figure 3.

Figure 3

Receptors involved in THC and CBD’s effect on lymphocyte proliferation. (A) Proliferation of CFSE-stained, 4 days CD3-activated, splenocytes from CB2 knockout mice was analyzed using flow cytometry. Summary of four independent experiments. The differences of CBD, THC BDS and CBD BDS as compared to control are significant starting from 3 µg/mL. The differences of THC as compared to control are significant starting from 10 µg/mL. The differences of THC when compared to CBD is significant starting from 3 µg/mL. (B) The influence of PPARγ antagonist, GW9662, on CBD’s effect on lymphocyte activation. Proliferation of CFSE-stained, CD3-activated murine splenocytes was analyzed using flow cytometry analysis. Summary of eight independent experiments. p Value—samples were compared to act spl + CBD (right) or act spl (left). (C) Real time PCR analysis for the expression of cyp1a1 in activated splenocytes treated with THC or CBD. Summary of four independent experiments. Results are expressed as mean + SEM. p Value *, <0.05; **, <0.001;, act spl: activated splenocytes, THC: D9 tetrahydrocannabinol, CBD: cannabidiol, BDS: Botanical Drug Substance.