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Effect of MID1PI1 depletion on proliferation and lipogenesis-related genes. (a) Expression level of MID1IP1 in different cell lines. β-actin was used as loading control. (b) Depletion level of MID1IP1 for 48 h in HepG2 cells by qRT-PCR. (c) Effect of MID1PI1 depletion on proliferation in HepG2 cells by MTT assay. (d,e) Effect of MID1PI1 depletion on the mRNA level of SREBP-1c and FAS in HepG2 cells by RT-qPCR analysis. All experiments were independently performed at least three times.
