Figure 6.

The root length of ABA treatment and expression level of ABA-responsive genes in transgenic lines. The six-day-old seedlings of WT and TaCOMT transgenic lines were transferred to ½ MS medium containing 0, 5, 10 and 20 µM ABA. A week later, the phenotype of roots was investigated (bar = 2 cm) (A). The total root length of WT and transgenic lines were statistically analysis (B) For the expression level of ABA-responsive genes, two-week-old Arabidopsis seedlings were placed on filter paper for two hours to simulate drought, and then the transcription level of ABA-responsive genes was analyzed (C). Expression of AtActin as a loading control. At least 25 seedlings per phenotype were counted. The data are shown as the means ± SDs (n = 25) of three experiments. ANOVA test demonstrated significant differences (* p < 0.05, ** p < 0.01).