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. 2019 Jan 28;19(3):539. doi: 10.3390/s19030539

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The enzymatic hydrolysis of ATP and ADP to AMP by apyrase. (a) The real-time measurement of 1.6 mM ATP and ADP (duplicate runs are shown for both ATP and ADP). (b) The linear fit of end-point values in refractive index units vs. initial substrate concentration. ATP (blue) R² > 0.99, slope 1.51 × 10⁻⁵ RIU/mM, ADP (green) R² > 0.99, slope 0.68 × 10⁻⁵ RIU/mM.