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. 2019 Feb 7;20(3):714. doi: 10.3390/ijms20030714

Figure 1.

Figure 1

AN7 treatment elevates Histone H3 acetylation levels in laser-applied eyes. Representative images of cryosections from naïve eyes (without laser applications) of mice treated by intraperitoneal (IP) AN7 or saline (A,B,E,F) and lesions sites of laser-applied eyes of mice treated with IP AN7 or saline (C,D,G,H), from day 7 post laser application. (A–D) Hematoxylin and Eosin (H&E). Scale bar, 100 µm. Yellow arrows mark the same blood vessel in the H&E and the corresponding immunostaining image to allow orientation. (E–H) Immunostaining for CD31 (green), acetylated histone H3 (AC-H3; red) and cells nuclei, DAPI (blue). GCL, Ganglion Cells Layer; INL, Inner Nuclear Layer; ONL, Outer Nuclear Layer; RPE, Retinal Pigmented Epithelium; C, Choroid. Scale bar, 50 µm. (I) Quantification of AC-H3 staining in laser applied eyes.