Figure 3.

Map-based cloning and identification of the YE1 gene. (A) The YE1 locus was mapped to the interval between molecular markers RM20223 and RM5371 on chromosome 6. (B) The YE1 locus was further delimited to a ~85 kb genomic region between markers ID4 and ID6. (C) The candidate region contained eleven ORFs. (D) The gene structure of candidate LOC_06g40080. The red arrow indicates the ye1 mutation at 158 nucleotide position in LOC_06g40080, which changes Ter at position 53 to a stop codon. The asterisk indicates the stop codon. (E) The identification of the YE1 gene in the ye1/WT F₂ population by sequencing.