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. 2019 Jan 26;20(3):523. doi: 10.3390/ijms20030523

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Basal human adipose derived stem cells (ADSCs) in vitro culture (non-induced). (B–D) human ADSCs induced with 0.5 μM AECK time points captured at 3, 5 and 24 h respectively. Time course shows minimal structural changes and some cellular contraction producing cells in (D) which are marginally more slender than those in (A). (E) Basal human ADSCs in vitro culture (non-induced). (F–H) human ADSCs induced with 0.6 μM lanthionine ketamine (LK) time points captured at 3, 5, and 24 h respectively. Time course adequately exhibits large structural reconfiguration of ADSCs during differentiation. Cells display bipolar elongation, process, and spindle formation. Microscopy images using a 10× objective lens; scale bar 100 μm. (I) The cell count for the time course of each treatment AECK (blue) and LK (red).