Figure 2.

Biological effects in an ex vivo SG model. (A) Ex vivo SG model experimental set up used for testing the cytotoxicity of pilocarpine and biocompatibility of different nanofiber mats. (B) Glands were treated with therapeutic concentrations of pilocarpine (Pilo) ranging from 0.1 to 20 µg/mL. All gland growth index values at day 1 and 3 were normalized to time 0 (baseline). (C) ATP activity (a readout for gland viability) three days after glands were treated with therapeutic concentrations of pilocarpine (0.1–20 µg/mL). Error bars represent SD from n = 4. * p ˂ 0.05 and ** p ˂ 0.01 when compared to positive control without pilocarpine (0 μg/mL). Negative control (-ve CTL) represent glands damaged by gamma radiation.