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. 2019 Jan 28;20(3):541. doi: 10.3390/ijms20030541

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Biocompatibility of pilocarpine-loaded and unloaded nanofiber mats (PNM and UNM, respectively) in the ex vivo SG culture model. (A) Bright field images of the growing SG glands at 3.2× magnification. Scale bar: 400 µm. (B) SG epithelial viability (readout for organ biocompatibility) was supported by unloaded nanofibers and by 0.5 mm and 1 mm loaded nanofibers. Y axis is a ratio of epithelial bud number at a specific culture time relative to baseline. Error bars represent SD from n = 4–12. * p ˂ 0.05, when compared to positive control without pilocarpine (+ve CTL) at every culture day; ns: not significant when compared to “+ve CTL”. Negative control (-ve CTL) represent glands damaged by gamma radiation. PNM: Pilocarpine nanofiber mats. UNM: unloaded nanofiber mats.