Table 1.

The quenching constant (K_sv), binding constant (K_b), binding stoichiometry (n), and thermodynamic parameters between HSA and CBH from fluorescence quenching experiments at 25, 30 and 37 °C.

Parameter	25 °C	30 °C	37 °C
n (binding stoichiometry, HSA:CBH)	0.99 ± 0.11	1.01 ± 0.13	1.101 ± 0.12
KSV (Stern-Volmer constant, M−1)	1.7 ± 0.21 × 104	1.3 ± 0.24 × 104	1.0 ± 0.31 × 104
Kb (binding constant, M−1)	1.6 ± 0.22 × 104	1.4 ± 0.19 × 104	1.2 ± 0.23 × 104
kq (bimolecular quenching rate constant, M−1 s−1)	2.6 ± 0.12×1012	2.2 ± 0.15 × 1012	1.7 ± 0.19 × 1012
ΔH° (binding enthalpy, kcal mol−1)	-	−4.44±0.16	-
TΔS° (entropy change, kcal mol-1)	1.28 ± 0.18	1.30 ± 0.15	1.33 ± 0.16
ΔG° (Gibbs free energy change, kcal mol-1)	−5.72 ± 0.12	−5.75 ± 0.15	−5.78 ± 0.14

The data are the means ± standard deviations of three independent trials.