Fig. 2.
Mechanical ventilation affects key cognition-relevant cytokine responses in the brain and plasma of WT and ADtg mice. The Meso Scale Discovery (MSD) multiplex inflammatory assay was performed on plasma and brain specimens from all experimental groups (n = 8–12 mice/group). a, b IL-6 expression. c Pearson’s r correlation analysis between cerebral and plasma IL-6 levels and d cerebral IL-6 and % PMNs in BAL. e, f TNF-α expression. g Correlations between cerebral and plasma TNF-α levels and h cerebral TNF-α and % PMNs in BAL. i, j IL-1β expression. k Correlations between cerebral and plasma IL-1β levels and l cerebral IL-1β and % PMNs in BAL. m IL-5 expression in the brain. n Correlation between brain IL-5 and % PMNs in BAL. o IL-10 expression in the brain. p Correlation between brain IL-10 and % PMNs in BAL. Data from individual mice and group means with standard error of measurements are shown as well as p values (pi = p value for interaction; pMV = p value for MV intervention effect; pg = p value for genotype effect); mice in the MV group are indicated by orange dots and mice in the SB group by yellow dots. Fold increase and percentage decrease between the groups are shown in red. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.0001, using two-way ANOVA with Holm-Sidak’s post hoc multiple comparisons correction or Pearson’s r correlation analysis with 95% CI