Fig. 2.
GmBZL3P219L rescues the bri1–5 insensitive phenotype in transgenic plants. GmBZL3/bri1–5 indicates overexpressed GmBZL3 in the bri1–5 mutant. GmBZL3#2–1-1, GmBZL3#1–1-4 and GmBZL3#4–1-4 are the three independent lines in which GmBZL3 is overexpressed in the bri1–5 background. GmBZL3P219/bri1–5 indicates overexpressed GmBZL3P219 in the bri1–5 mutant. GmBZL3P219/bri1–5#3–2-5, GmBZL3P219/bri1–5#5–2-5 and GmBZL3P219L/bri1–5#3–1-1 are the three independent lines that overexpress GmBZL3P219L in a bri1–5 background. a The plant height of a one-month-old bri1–5 mutant was significantly rescued by overexpressing GmBZL3P219. b-c Comparison of the length of the hypocotyl of 4-day seedlings (grown in the dark) in various plant lines. d-e Comparison of the length of the primary root of 7-day seedlings (grown under light) in various plant lines. f Transgene expression in various lines revealed by quantitative RT-PCR. Significant difference between the compared values are shown (P < 0.05). Error bars indicate S.D. (n ≥ 30)