Table 1.
Primers and probes used for PCV screening and sequencing
| Primer, probe | Sequence | Accession number | Position (nt - nt) | Length of the amplicon (bp) | Reference |
|---|---|---|---|---|---|
| PCV3_real_FW | AGTGCTCCCCATTGAACG | KT869077 | 1427–1444 | 135 | Palinski et al., 2016 [6] |
| PCV3_real_RV | ACACAGCCGTTACTTCAC | 1561–1544 | |||
| PCV3_real_probe | FAM-ACCCCATGGCTCAACACATATGACC-BHQ1 | 1473–1449 | |||
| PCV3_seq2_FW | GTCGTCTTGGAGCCAAGTG | 1609–1627 | 825 | Palinski et al., 2016 [6] | |
| PCV3_seq2_RV | CGACCAAATCCGGGTAAGC | 433–415 | |||
| PCV1 fw (F41) | ATACGGTAGTATTGGAAAGGTAGGG | 441–465 | 688 | Mankertz et al., 2000 [42] | |
| PCV1 rev (B42) | ACACTCGATAAGTATGTGGCCTTCT | 1129–1106 | |||
| PCV2 fw (F66) | GGTTTGTAGCCTCAGCCAAAGC | KT868491.1 | 567–546 | 416 | |
| PCV2 rev (B67) | GCACCTTCGGATATACTGTCAAGG | 152–175 |