Fig. 5.

LINC00707 negatively regulate miR-370-3p during osteogenic induction a mRNA levels of RUNX2, ALP, and OCN detected by qRT-PCR in HBMSCs co-transfected with LINC00707/NC and miR-370-3p mimics/mimics NC after osteogenic induction for 7 days. b mRNA levels of RUNX2, ALP, and OCN were detected by RT-PCR in HBMSCs co-transfected with sh-LINC00707/sh-NC and miR-370-3p inhibitor/inhibitor NC after osteogenic induction for 7 days. c Protein level of RUNX2 in HBMSCs co-transfected with LINC00707/NC and miR-370-3p mimics/mimics NC after osteogenic induction for 7 days. d Protein level of RUNX2 in HBMSCs co-transfected with sh-LINC00707/sh-NC and miR-370-3p inhibitor/inhibitor NC after osteogenic induction for 7 days. e, g Relative activity of ALP, ARS staining, and ALP staining in HBMSCs co-transfected with LINC00707/NC and miR-370-3p mimics/mimics NC after osteogenic induction for 7 days. f, h Relative activity of ALP, ARS staining, and ALP staining in HBMSCs co-transfected with sh-LINC00707/sh-NC and miR-370-3p inhibitor/inhibitor NC after osteogenic induction for 7 days. Data represent the mean ± S.D. *P<0.05, **P<0.01