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. 2019 Feb 22;24:15. doi: 10.1186/s11658-019-0142-4

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — HO-1 is a direct target of miR-218-5p. The conserved binding sites between miR-218-5p and HO-1 were predicted using online software TargetScan and miRDB (a), and the luciferase activity assay was performed to validate HO-1 as a direct target of miR-218-5p (b). After transfection with miR-218-5p mimics into GMCs, the mRNA (c) and protein (d) expression levels of HO-1 were measured by RT-qPCR and western blotting, respectively. Values are expressed as mean ± SEM, n = 3 in each group. ^* P < 0.05 versus control group