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. Author manuscript; available in PMC: 2019 Feb 23.
Published in final edited form as: Mitochondrion. 2017 Dec 20;44:20–26. doi: 10.1016/j.mito.2017.12.008

Fig. 1.

Fig. 1.

Development of pCAG-CAT-MitoTimer construct. A plasmid DNA containing conditional expression unit of MitoTimer was constructed by genetic engineering and used for conditional expression In cultured cells to confirm its inducibility. (a) A schematic presentation of the recombinant pCAG-CAT-Mitotimer construct generated by subcloning MitoTimer coding region Into a plasmid DNA containing the CMV enhanced β-actin promoter; (b) Representative confocal Images of C2C12 cells co-transfected of pCAG-CAT-MitoTimer with either empty vector pCI-neo or pCMV-Cre. MitoTimer expression was only detected In the presence of Cre; and (c) Genotyping of the F1 progeny following the crossbredding of CAG-CAT-MitoTimer mice with wild type (WT) mice. pCAG-CAT-MitoTimer and isolated DNA fragment of CAG-CAT-MitoTimer expression unit were Included to serve as positive controls 1 and 2 (PC1 and PC2), respectively.