Figure 1.

Nuclear sample preparation. A) Immunoblot analysis of different nuclei lysis conditions using the nuclear protein PARP1 and the chromatin-bound proteins CBX8 and TATA-binding protein (TPB) to assess lysis. B) Quantitation of the immunoblot normalized to radio-immumoprecipitation assay, a high detergent lysis buffer. C) Different buffer compositions tested. D) Immunoblot analysis of whole cell lysate (WCL), cytoplasmic, and nuclear fractions to demonstrate enrichment of nuclear proteins against lamin B1 (nuclear) and α-tubulin cytoplasmic antibodies.