Figure 4.
ILF2 enhances CREB protein stability. (a) The mRNA level of CREB was tested by qPCR in control and Bel-7402 or SMMC-7721 cells with knockdown and overexpression of ILF2. (b, c) CHX chase experiments were performed in control and Bel-7402 or SMMC-7721 cells with ILF2 knockdown, ILF2 overexpression, and ILF2 knockdown and ILF2 overexpression simultaneously. The images of ILF2, CREB, and GAPDH were performed by western blotting. The ratios between CREB and GAPDH were calculated with the 0 h point arbitrarily set to 100%.