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. Author manuscript; available in PMC: 2019 Feb 24.
Published in final edited form as: Biochim Biophys Acta Mol Cell Biol Lipids. 2018 Apr 18;1863(8):795–805. doi: 10.1016/j.bbalip.2018.04.010

Fig. 2. Inhomogeneous distribution of pneumolysin on the plasmalemma of perforated and non-perforated cells.

Fig. 2

Annexin A2-mCherry expressing SH-SY5Y and HEK293 cells were incubated with EGFP-PLY (29.1 nM) in the presence of 2.5 mM Ca2+. Note the cytosolic localization of the Ca2+ senor, AnxA2-mCherry (asterisks) in resealed cells; the plasmalemmal localization of AnxA2-mCherry (arrows) marks perforated cells. Representative images are shown. n = 4. Magnification bars: SH-SY5Y = 20 μm, HEK293 = 10 μm. Incubation time = mm:ss.