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. Author manuscript; available in PMC: 2019 Feb 24.
Published in final edited form as: Biochim Biophys Acta Mol Cell Biol Lipids. 2018 Apr 18;1863(8):795–805. doi: 10.1016/j.bbalip.2018.04.010

Fig. 8. Pneumolysin preferentially binds to the DPPC/cholesterol enriched phase on giant vesicles that feature a phase separated lipid bilayer.

Fig. 8

Single-plane confocal images of a vesicle composed of DOPC/DPPC/cholesterol (30:30:40) containing 0.2 mol/% NAP treated with mCherry-PLY (3.2 μM, red). NAP denotes the cholesterol-rich, lo domain (green). Representative images are shown. n = 3. Magnification bar = 10 μm.