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. Author manuscript; available in PMC: 2019 Feb 24.
Published in final edited form as: Biochim Biophys Acta Mol Cell Biol Lipids. 2018 Apr 18;1863(8):795–805. doi: 10.1016/j.bbalip.2018.04.010

Fig. 9. Pneumolysin preferentially binds to boundaries between cholesterol-rich and cholesterol-depleted phases of giant vesicles composed of phosphati-dylcholine and sphingomyelin.

Fig. 9

(a) Single-plane confocal images of a vesicle composed of DOPC/PSM/cholesterol (30:30:40) containing 0.2 mol/% NAP and treated with mCherry-PLY (3.2 μM, red). NAP denotes the cholesterol-rich, lo domain (green). Magnification bar = 10 μm. (b) Top view on the upper hemisphere of the giant vesicle shown in (a), at ~12 min PLY incubation time. Note the enhanced accumulation of PLY at the phase boundaries. n = 3. Magnification bar = 5 μm.