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. Author manuscript; available in PMC: 2020 Feb 15.
Published in final edited form as: Methods. 2018 Nov 27;155:131–139. doi: 10.1016/j.ymeth.2018.11.015

Figure 1. Experimental schematic.

Figure 1.

The experimental workflow of polysome profiling from mammalian tissues or isolated cell-types following by next-generation sequencing analysis. Either whole tissue or single cell-types are isolated from the animal. The cells are lysed, nuclei and cell debris are removed, and the cytoplasm is layered onto a sucrose gradient. Following centrifugation, the gradient is flowed through an absorbance detector and the profile is collected. mRNA can then be isolated from fractions for downstream analysis.