Table 3.
60% sucrose in polysome buffer
| Component | Final Concentration |
|---|---|
| Tris-HCl at pH 7.5 | 10 mM |
| KCl | 100 mM |
| MgCl2 | 5 mM |
| Sucrose w/v | 60% w/v |
| Molecular Grade H2O | -- |
Suggested preparation of 500 mL. Add 50 mL of 10X polysome buffer sto ck and 200 mL of ddH2O into a beaker with a hot plate with a stir bar to 80°C. Slowly add 300 g sucrose. Wait until the sucrose has completely dissolved and then let the solution cool with continued stirring. Fill to 500 mL with ddH2O and filter through a .22 μm filter. Store at 4°C for up to a month and check for any microbial growth before using.