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Hepatocyte perfusion solution B
| HBSS w/ phenol red | 1X |
| Cycloheximide | 300 μg/mL |
| CaCl2 | 5.4 mM |
| Soybean Trypsin Inhibitor | .04 mg/mL |
| Collagenase type I | 90 U/mL |
Supplement 1X HBSS (w/ phenol red) with cycloheximide, CaCl2, soybean trypsin inhibitor, and collagenase type I. Prepare fresh and warm to 39°C for perfusion.
