Figure 5:

Impact of varying parameters in the equations used for modeling RNA decay: For each panel, the left graph represents the shapes of several arbitrary exponential curves after perturbing a single parameter in each model. Here, the y-axis represents labeled RNA abundance relative to the equilibrium level of labeled RNA in label-containing media. The x-axis represents time in the number of half-lives for a single exponential curve with a decay rate of α₀, indicated with T_α0. The right graph in each panel represents half-lives calculated from each of the curves from the corresponding left graph in the same panel and relative to T_α0. In each graph, the equation used to either model the decay or determine the half-life is displayed. A) The effect of varying α on relative RNA abundance (left) and half-life (right) when modeling RNA decay with a single exponential. B) The effect of varying α when modeling RNA decay in a to equilibrium experimental design. C) The effect of varying β with a fixed c and a fixed α when modeling RNA decay with a two component mixed exponential. D) The effect of varying c with a fixed α and β when modeling RNA decay with a two component mixed exponential.