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. Author manuscript; available in PMC: 2020 Mar 15.
Published in final edited form as: Methods. 2018 Aug 24;157:56–65. doi: 10.1016/j.ymeth.2018.08.009

Figure 5: SMT of Halo-lamin A reveals compartment-specific differences in binding dynamics.

Figure 5:

(A) Relative ratios of diffuse, fast bound, and slow bound molecules (top) and the associated dwell times (bottom) of SMT data from Figure 4 where only the molecules located at the nuclear periphery were analyzed.

(B) Relative ratios of diffuse, fast bound, and slow bound molecules (top) and associated dwell times (bottom) of SMT data from Figure 4 where only the molecules in the nuclear interior were analyzed.

(C) The individual populations for each lamin mutation at the nuclear periphery from (A) plotted against the respective population in the nuclear interior from (B). Using each lamin mutant as a data point, linear regressions were fit to the relationship between nuclear compartments for each population: the ratio of diffuse, fast bound, and slow bound molecules as well as the fast and slow bound dwell times (top to bottom).