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. 2019 Feb 18;13:30. doi: 10.3389/fnbeh.2019.00030

Figure 1.

Figure 1

Limited bedding and nesting do not induce hypothermia or alter brown adipose tissue (BAT) thermogenesis. (A,B) Photographs of standard cages (Control; A) and limited bedding and nesting (LBN) cages (B). (C,D) Core (rectal) temperatures do not differ at the beginning of the LBN experience on P2 (C) or at the end of the LBN experience on P9/10 (D; t-test, p > 0.1). Data are mean ± SEM; n = 21–33/group. (E) Gross wet weights of BAT (subscapular depot) do not differ between groups, regardless of whether data are normalized by body weight (t-test, p > 0.1). Data here are normalized to body weight mean ± SEM; n = 4/group. (F) Core temperatures do not differ by caging condition at baseline on P9/10, or 5 min after the initiation of cold stress. Cold stress consisted of 5 min at 4°C, followed by immediate measurement of core temperature. Data are mean ± SEM; n = 10/group. No significant between-group differences by repeated-measures two-way ANOVA; p > 0.1; significant within-subjects effect of time, F(1,18) = 11.01, *p < 0.005. Note that data from male and female pups are combined here due to an absence of sex differences.